Sample Preparation

The test design, development, and validation are described in Grskovic et al., J Mol Diagn, 2016. Cell-free DNA is purified from plasma collected in Streck cell-free DNA BCT® tubes. Cell-free DNA extracted from plasma is used as the template in a pre-amplification PCR reaction with primer pairs that specifically amplify the genomic regions containing the AlloSure SNPs. The pre-amplification product is used as the template in parallel small-multiplex microfluidic PCR amplification library preparation and next generation sequencing.

For the complete AlloSure Specimen Collection and Shipping Information, please view the latest revision of the AlloSure Laboratory Services Guide by clicking here.

To view the Quick Instructions for AlloSure Blood Draw, click here.